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<h1 id="firstHeading" class="firstHeading mw-first-heading"><span class="mw-page-title-main">Sakaguchi-Test</span></h1>
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<p>Der <b>Sakaguchi-Test</b> ist ein <a href="Kolorimetrie" title="Kolorimetrie">kolorimetrisches</a> <a href="Analytische_Chemie" title="Analytische Chemie">Analyseverfahren</a> zur Bestimmung von <a href="Guanidin" title="Guanidin">Guanidinogruppen</a>, beispielsweise von <a href="Arginin" title="Arginin">Arginin</a> in <a href="Peptid" title="Peptid">Peptiden</a> und <a href="Protein" title="Protein">Proteinen</a>. Der Test wurde nach dem <a href="Japan" title="Japan">japanischen</a> <a href="Lebensmittelchemiker" title="Lebensmittelchemiker">Lebensmittelchemiker</a> Shoyo Sakaguchi (1900–1995) benannt, der ihn im Jahr 1925 veröffentlichte.<sup id="cite_ref-Sakaguchi1925_1-0" class="reference"><a href="#cite_note-Sakaguchi1925-1"><span class="cite-bracket">[</span>1<span class="cite-bracket">]</span></a></sup>
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<div class="mw-heading mw-heading2"><h2 id="Eigenschaften">Eigenschaften</h2></div>
<p><a href="Reagenz" class="mw-redirect" title="Reagenz">Reagenzien</a> des Sakaguchi-Tests sind <a href="1-Naphthol" title="1-Naphthol">1-Naphthol</a> und <a href="Natriumhypobromit" title="Natriumhypobromit">Natriumhypobromit</a>. Vorhandene Guanidinogruppen reagieren damit zu einem roten <a href="Farbstoff" class="mw-redirect" title="Farbstoff">Farbstoff</a>.<sup id="cite_ref-2" class="reference"><a href="#cite_note-2"><span class="cite-bracket">[</span>2<span class="cite-bracket">]</span></a></sup><sup id="cite_ref-3" class="reference"><a href="#cite_note-3"><span class="cite-bracket">[</span>3<span class="cite-bracket">]</span></a></sup><sup id="cite_ref-4" class="reference"><a href="#cite_note-4"><span class="cite-bracket">[</span>4<span class="cite-bracket">]</span></a></sup><sup id="cite_ref-5" class="reference"><a href="#cite_note-5"><span class="cite-bracket">[</span>5<span class="cite-bracket">]</span></a></sup>
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<div class="mw-heading mw-heading2"><h2 id="Einzelnachweise">Einzelnachweise</h2></div>
<ol class="references">
<li id="cite_note-Sakaguchi1925-1"><span class="mw-cite-backlink"><a href="#cite_ref-Sakaguchi1925_1-0">↑</a></span> <span class="reference-text">Shoyo Sakaguchi: <cite style="font-style:italic">Über eine neue Farbenreaktion von protein und arginin</cite>. In: <cite style="font-style:italic"><a href="Journal_of_Biochemistry" title="Journal of Biochemistry">Journal of Biochemistry</a></cite>. 5. Jahrgang. <a href="Oxford_University_Press" title="Oxford University Press">Oxford University Press</a>; Japanese Biochemical Society, April 1925, <span style="white-space:nowrap">S.<span style="display:inline-block;width:.2em"> </span>25–31</span>, <a href="Digital_Object_Identifier" title="Digital Object Identifier">doi</a>:<span class="uri-handle" style="white-space:nowrap"><a rel="nofollow" class="external text" href="https://doi.org/10.1093/oxfordjournals.jbchem.a128359">10.1093/oxfordjournals.jbchem.a128359</a></span> (<a rel="nofollow" class="external text" href="https://academic.oup.com/jb/article-abstract/5/1/25/2183654">oup.com</a>).<span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Abook&rfr_id=info:sid/de.wikipedia.org:Sakaguchi-Test&rft.atitle=%C3%9Cber+eine+neue+Farbenreaktion+von+protein+und+arginin&rft.au=Shoyo%26%2332%3BSakaguchi&rft.btitle=Journal+of+Biochemistry&rft.date=1925-04&rft.doi=10.1093%2Foxfordjournals.jbchem.a128359&rft.genre=book&rft.pages=25-31&rft.pub=Oxford+University+Press%3B+Japanese+Biochemical+Society&rft.volume=5.+Jahrgang" style="display:none"> </span></span>
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<li id="cite_note-2"><span class="mw-cite-backlink"><a href="#cite_ref-2">↑</a></span> <span class="reference-text"><span class="book">Chatterjea: <cite class="lang" lang="en" dir="auto" style="font-style:italic">Textbook of Biochemistry for Dental/Nursing/Pharmacy Students</cite>. Jaypee Brothers Publishers, 2004, ISBN 978-81-8061-204-6, <span style="white-space:nowrap">S.<span style="display:inline-block;width:.2em"> </span>51</span> (englisch, <a rel="nofollow" class="external text" href="https://books.google.com/books?id=xN0YYypnZVkC&pg=PA51">google.com</a>).<span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Abook&rfr_id=info:sid/de.wikipedia.org:Sakaguchi-Test&rft.au=Chatterjea&rft.btitle=Textbook+of+Biochemistry+for+Dental%2FNursing%2FPharmacy+Students&rft.date=2004-01-01&rft.genre=book&rft.isbn=9788180612046&rft.pages=51&rft.pub=Jaypee+Brothers+Publishers" style="display:none"> </span></span></span>
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<li id="cite_note-3"><span class="mw-cite-backlink"><a href="#cite_ref-3">↑</a></span> <span class="reference-text"><span class="book">R. A. Joshi: <cite class="lang" lang="en" dir="auto" style="font-style:italic">Question Bank of Biochemistry</cite>. New Age International, 2006, ISBN 978-81-224-1736-4, <span style="white-space:nowrap">S.<span style="display:inline-block;width:.2em"> </span>64</span> (englisch, <a rel="nofollow" class="external text" href="https://books.google.com/books?id=Acf9BkEqJWYC&pg=PA64">google.com</a>).<span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Abook&rfr_id=info:sid/de.wikipedia.org:Sakaguchi-Test&rft.au=R.+A.+Joshi&rft.btitle=Question+Bank+of+Biochemistry&rft.date=2006&rft.genre=book&rft.isbn=9788122417364&rft.pages=64&rft.pub=New+Age+International" style="display:none"> </span></span></span>
</li>
<li id="cite_note-4"><span class="mw-cite-backlink"><a href="#cite_ref-4">↑</a></span> <span class="reference-text"><span class="book">Dandekar: <cite class="lang" lang="en" dir="auto" style="font-style:italic">Practicals And Viva In Medical Biochemistry</cite>. Elsevier India, 2004, ISBN 978-81-8147-025-6, <span style="white-space:nowrap">S.<span style="display:inline-block;width:.2em"> </span>28</span> (englisch, <a rel="nofollow" class="external text" href="https://books.google.com/books?id=s9HEoJquruwC&pg=PA28">google.com</a>).<span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Abook&rfr_id=info:sid/de.wikipedia.org:Sakaguchi-Test&rft.au=Dandekar&rft.btitle=Practicals+And+Viva+In+Medical+Biochemistry&rft.date=2004-01-01&rft.genre=book&rft.isbn=9788181470256&rft.pages=28&rft.pub=Elsevier+India" style="display:none"> </span></span></span>
</li>
<li id="cite_note-5"><span class="mw-cite-backlink"><a href="#cite_ref-5">↑</a></span> <span class="reference-text"><span class="book">Rashmi A. Joshi, Victor Marks: <cite class="lang" lang="en" dir="auto" style="font-style:italic">Guide to Bio-chemistry</cite>. B. Jain Publishers, 2004, ISBN 978-81-8056-053-8, <span style="white-space:nowrap">S.<span style="display:inline-block;width:.2em"> </span>74</span> (englisch, <a rel="nofollow" class="external text" href="https://books.google.com/books?id=avDlDrPR4joC&dq=The+guanidine+group+in+arginine+reacts+with+the+Sakaguchi+reagent+to+form+a+red-coloured+complex&pg=PA74">google.com</a>).<span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Abook&rfr_id=info:sid/de.wikipedia.org:Sakaguchi-Test&rft.au=Rashmi+A.%26%2332%3BJoshi%2C%26%2332%3BVictor%26%2332%3BMarks&rft.btitle=Guide+to+Bio-chemistry&rft.date=2004-06-30&rft.genre=book&rft.isbn=9788180560538&rft.pages=74&rft.pub=B.+Jain+Publishers" style="display:none"> </span></span></span>
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